Serum Free T-Cell Expansion Medium 003 - - Cells Reagents - Shanghai Weike Biotechnology Co.,Ltd.

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Serum Free T-Cell Expansion Medium 003

NobimpexBrand：
N103-1000Cat No：
1LSize：
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Serum-free T-Cell Expansion Medium has been developed to promote the optimal expansion of T-Cells of human origin.
This medium supports high viable cell densities. The elimination of serum reduces performance variability in the medium and eliminates safety risks associated with possible adventitious agents in serum.

Description：

Serum Free T-Cell Expansion Medium 003，without L-glutamine，used to promote the optimal expansion of T-Cells

Advantages：

Serum-free T-Cell Expansion Medium is a proprietary formulation. The medium does not contain antibiotics or cytokines.

Human serum albumin and human transferrin are the only human origin materials and are non-reactive (donor level) for anti-HIV 1 & 2, anti-HCV and HB s Ag. Handle as if potentially infectious.

Storage and Stability：

2-8℃

Application：

This medium is supplied as a sterile (1X) liquid and must be supplemented with stimulatory antibodies, cytokines and/or antibiotics, if desired. Serum-free T- Cell Expansion Medium must also be supplemented With L-glutamine. Add 20 ml of 200mM L-glutamine solution or 0.584 g powder (irradiated) per liter of medium.

Procedure:
Plating Cultures
1. Prepare either fresh or frozen PBMNCs (peripheral blood mononuclear cells) as directed by the supplier or in accordance with established protocols.
2. Count cells using a hemacytometer.

3. Transfer the proper number of cells to the desired culture vessel containing medium supplemented with cytokines and stimulatory antibodies (and antibiotics if desired).
4. Place the culture vessel in a humidified incubator at 37°C and 5% CO2.

Product Profile:
Nobimpex’s Serum-free T-Cell Expansion Medium (Product number N103-1000) demonstrated rigorous expansion of T-cells from PBMNCs. This product was compared with several other commercially available serum-free expansion media for their ability to expand T-cells in T75 culture flasks. For these small-scale experiments, 200,000 PBMNCs/ml were incubated for up to 7 days in Serum-free medium or other commercial product containing 100 IU/ml IL-2 and anti-CD3 (OKT3, 20ng/ml) antibody.

The expanded T-Cell population from these experiment was then used in 51chromium release assays to test for functional cytolytic potential. Briefly, target cells (K562, a human chronic myelogenous leukemia cell line), were labeled with 51 Chromium and linked to anti-CD3 (OKT3) via Fc receptor. When mixed with effector or cytolytic T-cells, the target cells undergo apoptosis or lysis and release 51Chromium. The amount of 51chromium release into the supernatant is proportional to the number of targets killed and the number of functional cytolytic T-Cells.

Product Literature：

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